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  eBlot蛋白轉印系統-10分鐘輕松完成。提供樣機  
 


產品名稱:  eBlot蛋白轉印系統-10分鐘輕松完成。提供樣機
產品編號:  N201367182518
發布日期:  2014-9-20
查看次數:  293 
  產 品 詳 細 介 紹



eBlotTM 蛋白轉印系統
是一個高效的蛋白快速轉印系統,可以實現在7-10分鐘的時間內穩定、高效、快速、可靠地對各種類型的凝膠進行蛋白質印跡(Western Blot)實驗。eBlotTM 蛋白轉印系統包括eBlotTM 蛋白轉印儀、eBlotTM蛋白轉印試劑盒兩部分。配合試劑盒內含有特定緩沖液的正負極濾紙,eBlotTM蛋白轉印儀可在儀器的正極和負極之間形成一個勻強電場。在電場驅動下,凝膠中帶有負電荷的蛋白質分子發生定向移動,從而快速的轉移到NC或PVDF膜上,完成轉印過程。
與傳統的濕式轉印、半干式系統相比eBlotTM 蛋白轉印系統具有顯著的優點:
1. 快速高效,可在7-10分鐘內完成轉印過程
2. 操作簡便,人性化的流程設計方便實驗人員操作,減少人為操作失誤
3. 不含甲醇等有害成分
4. 適用性強,可用于不同類型的聚丙烯酰胺凝膠
5. 檢測靈敏度高,優于傳統濕法轉印

 

 
The eBlot ® Protein Transfer System is based on the proprietary fast semi-dry blotting technique developed by GenScript. To use the eBlot ® Protein Transfer System for rapid protein blotting, assemble the eBlot ® Protein Transfer Pad with your pre-run gel and the membrane on the eBlot ® Protein Transfer Device. A certain definite voltage between electrodes allows for rapid and directional movement of negatively charged protein molecules from the gel matrix onto the membrane within 7-10 minutes.
protein gel staining Figure 1. System Mechanism
The eBlot ® Cathode Pad andeBlot ® Anode Pad act as ion reservoirs that contain the appropriate anode and cathode buffers. A certain definite voltage between electrodes drive a rapid and directional movement of negatively charged protein molecules from the gel matrix onto the membrane.

  Shortened workflow increases your productivity  
   
The eBlot ® Protein Transfer System is faster and provides superior transfer quality compared to the conventional semi-dry western transfer and wet transfer systems (Table 1, Figure 2 and 3).
Table 1. Fast protein transfer with the eBlot ®
eblot system

  High and consistent protein transfer efficiency  
   
In addition to ease and convenience, the eBlot ® Protein Staining System provides higher detection sensitivity comparable to that of traditional protein transfer methods (Figure 3).
eblot transferred      Figure 2. Higher transfer quality of eBlot ® Protein Transfer System
(A) Comparison of protein transfer between eBlot® Protein Transfer System and a semi-dry western transfer method. (B) Comparison of protein transfer between eBlot ® Protein Transfer System and a tank wet western transfer method. Proteins were separated with Genscript Express™ PAGE Gels 8-16% and transferred to nitrocellulose . (A) Lanes 1-5 and 6-10: 0.313 µl, 0.625 µl, 1.25 µl, 2.5 µl, and 5.0 µl Genscript EasyWestern Protein Standard (22, 40, 60, 85, and 120 kDa); (B) Lanes 1-5 and 6-10: 5.0 µl, 2.5 µl, 1.25 µl, 0.625 µl, and 0.313 µl Genscript High Range EasyWestern Protein Standard (60, 85, 120, 160 and 200 kDa).
  Optimized self-contained system offers easy and convenient procedures  
   
The eBlot ® Protein Transfer System is created as a self-contained system. All you need to do is to simply assemble the eBlot ® Protein Transfer Pads and membrane together with the pre-run gel, then press the run button (Figure 3). 

eblot Protein transfer System
Figure3. eBlot ® Protein Transfer System



  Suitable for various types of mini gels  
   
The eBlot ® Protein Transfer System is designed to work with multiple types of mini polyacrylamide gels, including Tris-Glycine, Bis-Tris, Tris-Tricine, and Tris-Acetate gels, etc.

       protein gel staining
       protein gel staining
Available Video
eBlot™ Protein Transfer System
 Watch the video and see the  eBlot ® System in action
eBlot ® Resources
 eBlot ® User Manual
 eBlot ®Quick Reference Card 
 eBlot ® FAQ
 eBlot ® Flyer 
 eBlot ® Troubleshooting
Promotion
 Buy 1 get 6, Free consumbles for 4 months
 Free Trial Program 

 

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 寶林科(北京)生物科技有限公司

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 郵箱:Email:[email protected]   

 網址:http://www.ssehmw.live

 

 
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